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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>Hi
all,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>I’m
trying to use the gff pass through method to add the transcript/exon data
generated by Cufflinks as EST information. Maker works fine if I use a
fasta file for the EST input, but dies (with “Bus error”) if I try
to use a gff3 file that I’ve built from the Cufflinks gtf output and
validated with the on-line gff3 validator. I was wondering: 1). if
the gff pass through files referred to in the Maker tutorial (pass.tar.gz)
might be available to test our set up (running on Solaris); 2). whether anyone
has any bright ideas on why maker would be crashing only with the gff input.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>Thanks,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>Mike<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>-----------------------------------------------------<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>Michael Reith<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>Principal Research Officer</span></font><font
face="Times New Roman"><span style='font-family:"Times New Roman"'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>Functional Genomics Group Leader<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>NRC Institute for Marine Biosciences<o:p></o:p></span></font></p>
<p class=MsoNormal><st1:Street w:st="on"><st1:address w:st="on"><font size=2
face=Arial><span style='font-size:10.0pt'>1411 Oxford St</span></font></st1:address></st1:Street><font
size=2><span style='font-size:10.0pt'>.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=ES style='font-size:10.0pt'>Halifax,
N.S. B3H 3Z1<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=ES style='font-size:10.0pt'>Canada<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span lang=ES style='font-size:10.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>phone: (902)
426-8276<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>fax: (902)
426-9413<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt'>email: michael.reith@nrc.ca<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Verdana><span style='font-size:10.0pt;
font-family:Verdana'>-----------------------------------------------------------<br>
</span></font><font size=1 face=Verdana><span style='font-size:7.5pt;
font-family:Verdana'>The information contained in this e-mail may contain
confidential information intended for a specific individual and purpose. The
information is private and is legally protected by law. If you are not the
intended recipient, you are hereby notified that any disclosure, copying,
distribution or the taking of any action in reliance on the comments of this
information is strictly prohibited. If you have received this communication<br>
in error, please notify the sender immediately by telephone or return e-mail. <br>
Thank you.<br>
<br>
</span></font><font size=1 face=Verdana><span style='font-size:7.5pt;
font-family:Verdana'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=3 face=Arial><span style='font-size:12.0pt'><o:p> </o:p></span></font></p>
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